Paridhi Gupta | Senior Research Executive | Molecular and Cell Biology | Drug Discovery Experienced in in-vitro biology, gene manipulation, and oncology research. 🔬 In-vitro Biologist | Sun Pharma Advanced Research Company | Vadodara, India April 2022 - Present Conducting experiments using recombinant DNA technology to engineer cell lines, screening NCEs in vitro, troubleshooting assays, and executing experiments with scientific precision. Skilled in cell culture techniques, molecular biology methodologies, and data analysis. Contributing to the development of effective treatments for cancer. 🔬 Cell Line Development | Syngene International Pvt. Ltd. | Bangalore, India April 2019 - March 2022 Building gene manipulations (overexpression, knockdown, knockout, and knock-in) in various cell lines to observe their effects on cell physiology. Proficient in viral transduction techniques, liposome-based transfection, and CRISPR/Cas9 gene editing. Skilled in luminescent and fluorescent assays, flow cytometry, western blotting, and qPCR. 🔬 Master’s thesis| Indian Institute of Technology | Kanpur, India Investigated the role of SND1 as an oncogene in prostate cancer. Conducted stable SND1 knockdown in Prostate Cancer cell lines and analyzed oncogenic behavior through proliferation, foci formation, invasion, and migration assays. Identified signaling pathways affecting cholesterol metabolism and androgen synthesis. Explored transcriptional regulation mechanisms involving FOXA1 and AR. 🔬 Bachelor’s Thesis | Institute of Genomics and Integrative Biology | New Delhi, India June 2015 - December 2015 Cloned, expressed, and purified the human estrogen receptor ligand binding domain in a bacterial system. Explored an alternative to conventional antibodies for detecting ERα in breast cancer. Contributed to the development of efficient and low-cost diagnostic methods. 🎓 Education Master of Science in Molecular Biology | Indian Institute of Technology, Kanpur | 2018 Bachelor of Science in Biotechnology | Banasthali University | 2016 🏆 Skills Cell Culture | Molecular Biology | Gene Manipulation | PCR | CRISPR/Cas9 | Western Blotting | qPCR | Flow Cytometry | Data Analysis | Research Methodologies | Project Management 🎯 Passionate about making a difference in cancer research, improving patient outcomes, and translating scientific discoveries into clinical applications. Open to collaboration and new research opportunities. Let's connect and drive innovation in the field of oncology research!